Rapid communication: sequence of a quail calpain cDNA.
نویسندگان
چکیده
Name of sequence. Quail calpain cDNA. Species. Coturnix coturnix japonica. Origin of clone. Six primers (primers 1, 2, 3, 4, 5, and 6) complementary to highly conserved regions of the / m-calpain cDNA in skeletal muscle of chicken sequence were designed from chicken calpain 80-K subunit cDNA sequence (Ohno et al., 1984) and used in a reverse transcriptase polymerase chain reaction to amplify a product (about 2 kb) of the calpain 80-K subunit from quail muscle total RNA. The sequences of primers were designed to amplify five overlapping products that produce a 2.1kb consensus sequence as follows:
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ورودعنوان ژورنال:
- Journal of animal science
دوره 79 3 شماره
صفحات -
تاریخ انتشار 2001